教授
深溝 慶(ふかみぞ たもう)
【略歴】
昭和58(1983)年3月：九州大学農学部農芸化学科卒業
博士後期課程修了(農学博士号取得)
昭和58(1983)年4月：米国カンサス州立大学生化学教室博士研究員(～昭和60(1985)年)
昭和60(1985)年4月：近畿大学農学部助手
平成元(1989)年7月：近畿大学農学部講師)
平成4(1992)年4月：近畿大学農学部助教授
平成8(1996)年9月：カナダカルガリー大学に留学（～平成9年8月）
平成11(1999)年4月：近畿大学農学部教授
【学位】 農学博士(九州大学)
【専門分野】 酵素化学、タンパク質科学、生体物理化学
【研究内容】(全体の流れはこちらをご覧下さい)
キーワード：植物キチナーゼ、微生物キトサナーゼ、タンパク質工学、キチンキトサン、オリゴ糖、有用糖質生産
2) 植物キチナーゼの生理機能解析
3) 放線菌キトサン加水分解酵素の構造と機能
4) グース型リゾチームの反応機能解析
5) キチンキトサン多糖からの有用糖質生産
【所属学会】
★日本農芸化学学会
★日本キチンキトサン学会
★日本生化学会
★日本生物物理学会
★日本植物生理学会
★日本応用糖質科学会
【研究会活動】
★食品酵素化学研究会
【授業担当科目】
１年生：基礎ゼミ (前期)、生物化学Ⅰ(後期)、生物有機化学実験 (後期)
２年生：生体物理化学(前期)、酵素タンパク質工学(前期)、バイオサイエンス専門実験(前期)
３年生：バイオマテリアル実験Ⅰ・Ⅱ(前後期)、専門英語Ⅰ・Ⅱ(前後期)
４年生：卒業研究、専門演習Ⅰ・Ⅱ(前後期)
大学院：タンパク質化学工学特論、バイオマテリアル実験Ⅰ・Ⅱ、バイオマテリアル演習、バイオマテリアル特別演習、バイオマテリアル特別実験研究
【趣味】
昔は、よく山に登ってたんですが（冬山も）、今はなかなか、、、ですなぁ。しかし、そのうちにいつかはと、、ピッケルとアイゼンはうちの収納庫にしまってあります。
スポーツのビッグイベントは、ほとんど見ます。見ながら、涙が出てくることもあります。最近、涙したのは、2006年片岡（阪神）の引退セレモニー、2006年ラグビー日本選手権（早稲田－トヨタ戦）、、、ですかねぇ?
【抱負】
真面目だけがとりえですので、そのようにこれからもやっていきます。「塵である人間にいったい何ができようか」
【座右の銘】
「塵である人間にいったい何ができようか」
タイ東北部の河畔にて 南フランスにて
【著書】(6件)
Chitin/chitosan oligosaccharides: effective substrates for functional analysis
of chitinases/chitosanases, Ohnuma T and Fukamizo T, in Chitin, Chitosan, Oligosaccharides and Their Derivatives: Biological
Activities and Applications (ed. by Se-Kwon Kim), CRC press, 2010, in press
Enzymes Involved in Chitin and Chitosan Decomposition and Synthesis, Tamo Fukamizo, in Material Science of Chitin and Chitosan (ed. by Uragami, T. &
Tokura, S.), Kodansha, Tokyo, pp. 81-112
管理栄養士国家試験問題解説2005, 深溝 慶,11章
生化学を担当, メディック・メディア, 461-548
(2005)
放線菌キトサナーゼの構造と機能, 深溝 慶, 食品酵素化学の最新技術と応用―フードプロテオミクスへの展望―（監修、井上国世）シーエムシー出版, 70-83
(2004)
リゾチームとキチナーゼ, 深溝 慶, 酵素 4.3
ヒドロラーゼ, 廣川タンパク質化学, 第4巻, 廣川書店, 85-91
(2004)
【総説】(35件)
大沼貴之，翁長彰子，深溝 慶，平良東紀 : 特異な糖質結合モジュールLysMドメインの構造と機能． 化学と生物，(47)，676－677 (2009)
深溝 慶，大沼貴之 : 糖質加水分解酵素における反応解析法の進展． 化学と生物， （47）， 404－411 (2009)
深溝 慶, 田茂井政宏 : 環境ストレスに応答する植物由来キチナーゼとグルカナーゼ, 化学と生物, 45(10), 689-695 (2007)
Fukamizo, T., Yoshikawa, T., Katsumi, T., Amano, S., Saito, J., Miki, K., Ando, A., Brzezinski, R.: Substrate-Binding Mode of Bacterial Chitosanases. J. Appl. Glycosci. 52, 183-189 (2005)
深溝 慶: キトサンと食品タンパク質の相互作用, New Food Industry, 45, 7-12 (2003)
Tamo Fukamizo, Mechanism of chitosanase-substrate interaction, in Advances in Chitin Science, vol. VII, (ed. by Boucher, I., Jamieson, K., and Retnakaran, A.), pp. 101-104 (2003)
深溝 慶, 佐々木千絵, 田茂井政宏:植物キチナーゼ：構造と機能そして生理的役割:Food and Food Ingredients Journal of Japan, 208, 631-638 (2003)
Fukamizo, T., Defaye, J., McIntyre, D., and Vogel, H.J. b-1,4-linked 2-amino-2-deoxy-D-glucopyranosyl fluoride oligosaccharides; a novel substrate for chitosanase, in Advances in Chitin Science, vol. VI, (ed. by Varum, K.M., Domard, A., and Smidsrod, O.), pp. 125-128 (2002)
Sasaki, C., Kristiansen, A., Fukamizo, T., and Varum, K.M.. Fractionation of chitosan by biospecific adsorption to immobilized lysozyme, in Advances in Chitin Science, vol. VI, (ed. by Varum, K.M., Domard, A., and Smidsrod, O.), pp. 149-152 (2002)
Honda, Y., Kitaoka, M., Tokuyasu, K., Fukamizo, T., and Hayashi, K. Studies on substrate recognition of chitinases A and B from Serratia marcescence by using derivatives of 4-methylumbelliferyl chitobioside, in Advances in Chitin Science, vol. VI, (ed. by Varum, K.M., Domard, A., and Smidsrod, O.), pp. 239-240 (2002)
佐々木千絵, 深溝 慶：キチナーゼ･キトサナーゼによる食品素材オリゴ糖の生産、New Food Industry 43, 7-14 (2001)
深溝 慶, Juffer, A.H., and Brzezinski, R.: 酵素触媒反応を背後から支援する機能構造。蛋白質 核酸 酵素 46, 1261-1267 (2001)
深溝 慶, 佐々木千絵, 小島美紀：キチナーゼ・キトサナ－ゼの構造生物学―新しい酵素応用の可能性―．化学と生物 39, 377-383 (2001)
Fukamizo, T., Comparative enzymology of chitinases from plants and microbes, in Chitin Enzymology 2001 (ed. by Muzzarelli, R.A.A.), ATEC, Italy, pp. 213-217 (2001)
Kojima, M., Yoshikawa, T., Sutrisno, A., Ueda, M., Miyatake, K., Arai, M., and Fukamizo, T., Family 19 chitinase from Aeromonas sp. No.10S-24: enzymatic and structural properties of the catalytic domain, in Chitin Enzymology 2001 (ed. by Muzzarelli, R.A.A.), ATEC, Italy, pp. 323-327 (2001)
Sasaki, C., Takehara, H., Kuhara, S., Itoh, Y., and Fukamizo, T., Structure and substrate binding mode of family 19 chitinase from rice, Oryza sativa L., in Chitin Enzymology 2001 (ed. by Muzzarelli, R.A.A.), ATEC, Italy, pp. 329-334 (2001)
Fukamizo, T., Honda, Y., Juffer, A.H., Vogel, H.J., Tremblay, H., Boucher, I., Neugebauer, W.A., Brzezinski, R. Dissection of the catalytic cleft of chitosanase from Streptomyces sp. N174. in Chitin and Chitosan in Life Sciences (ed. by Uragami, T., Kurita, K., and Fukamizo, T.) Kodansha Scientific Ltd., Tokyo, pp. 411-414 (2000)
Sasaki, C., Yokoyama, A., Itoh, Y., and Fukamizo, T. Comparative enzymology of family 18 chitinases from rice (Oryza sativa, L.) and fungus (Coccidioides immitis). in Chitin and Chitosan in Life Sciences (ed. by Uragami, T., Kurita, K., and Fukamizo, T.) Kodansha Scientific Ltd., Tokyo, pp. 434-435 (2000)
Yamaguchi, T., Tremblay, H., Fukamizo, T., Brzezinski, R. An important role of Asp57 in substrate binding of chitosanase from Streptomyces sp. N174. in Chitin and Chitosan in Life Sciences (ed. by Uragami, T., Kurita, K., and Fukamizo, T.) Kodansha Scientific Ltd., Tokyo, pp. 458-459 (2000)
Ueda, M., Araki, K., Mitsuda, M., Sutrisno, A., Kojima, M., Kawaguchi, T., Fukamizo, T., Miyatake, K., and Arai, M. Cloning, nucleotide sequence, and expression of the family 19 chitinase gene (pRYA1 ORF) from Aeromonas sp. No.10S-24. in Chitin and Chitosan in Life Sciences (ed. by Uragami, T., Kurita, K., and Fukamizo, T.) Kodansha Scientific Ltd., Tokyo, pp. 464-465 (2000)
Fukamizo, T., Kinami, M., and Goto, S. Kinetic analysis of lDNA digestion catalyzed by restriction endonuclease, EcoRI: Effect of anti-DNA antibody on the kinetic constants. Mem. Fac. Agr. Kinki Univ. 33, 1-10 (2000)
Fukamizo, T., Yamaguchi, T., Tremblay, H., and Brzezinski, R. Substrate binding mechanism of chitosanase from Streptomyces sp. N174, in Advances in Chitin Science, vol. IV, (ed. by Peter, M.G., Domard, A., and Muzzarelli, R.A.A.), pp. 575-581 (1999)
Honda, Y., Kirihata, M., Fukamizo, T., Kaneko, S., Tokuyasu, K., and Brzezinski, R. Chitosanase-catalyzed hydrolysis of 4-methylumbelliferyl b-chitotrioside, in Advances in Chitin Science, vol. IV, (ed. by Peter, M.G., Domard, A., and Muzzarelli, R.A.A.), pp. 582-587 (1999)
Fukamizo, T., Honda, Y., Tremblay, H., and Brzezinski, R. An a-helical structure participating in the substrate binding of barley chitinase and Streptomyces sp. N174 chitosanase. in Genetics, Biochemistry, and Ecology of Cellulose Degradation. (ed. by Ohmiya, K., Hayashi, K., Sakka, K., Kobayashi, Y., Karita, S., and Kimura, T.) UNI publishers Co Ltd., Tokyo, pp. 695-702 (1998)
深溝 慶 ：酵素反応における相補性について．生物科学 47(4), 191-197(1996)
深溝 慶，本多 裕司 ：Boucher, I. and Brzenzinski, R.：放線菌由来のキトサナーゼの構造と機能．応用糖質科学 43(2), 247-256 (1996)
Fukamizo, T.
Interaction of partially acetylated chitotrisaccharides with hen egg white lysozyme. in Chitin Enzymology, (ed. by Muzzarelli, R.A.A.)
Eur. Chitin Soc., pp. 211-216 (1993)
Fukamizo, T., Goto, S., Ohtakara, A., Mitsutomi, M.
Structual Analysis of Partially Deacetylated Chitooligosaccharides by FAB-MS and NMR Spectroscopy. In Chitin Derivatives in Life Sciences. (ed. By Tokura and Azuma)
Japanese Society for Chitin/Chitosan 145-151 (1992)
Chaiwat, Fukamizo, T., Nelson, H. E., Toyoda, H., Ouchi, S.
Chemical Structure of Chitinous Components in mycelial wall of Fusarium species as a signal factor detemining host plant response. In Plant Cell Walls as Biopolymers with Physiological Functions (ed. By Masuda)
Yamada Science Foundation, Osaka 422-424 (1992)
Ouchi, S., Toyoda, H., Morimoto, Kurusu, Matsuda, Y., Goto, S., Fukamizo, T.
Integration of chitin degrading microbes into biological control system for Fusarium wilt of strawberry. in Biological Control of Plant Diseases. Progress and Challenge in the Future (ed, by Tjamos)
Plenum Press, New York 335-339 (1992)
Fukamizo, T., Ohkawa, T., Goto, S.
Theoretical analysis of the reaction-catalyzed by binary enzyme system of endo- and exo-spitting chitinases
Mem. Fac. Agr. Kinki Univ. 25, 1-7 (1992)
深溝 慶，後藤 幸男
リゾチーム触媒反応の温度依存性
近畿大学環境科学研究所研究報告 17, 173-178 (1989)
深溝 慶，後藤 幸男
リゾチーム触媒反応に対する色素ビーブリッヒスカーレットの影響
近畿大学環境科学研究所研究報告16, 181-187 (1988)
深溝 慶，後藤 幸男
キトオリゴ糖に対するリゾチームの加水分解効率
近畿大学環境科学研究所研究報告15, 141-148 (1987)
Kuhara, S., Iwamoto, S., Yanase, Y., Fukamizo, T., Hayashi, K.
Note on the separation of reacting system by chromatography
J. Fac. Agric. Kyusyu Univ. 27, 33-45 (1982)
【論文】(79件)
Taira T, Fujiwara M, Dennhart N, Hayashi H, Onaga S, Ohnuma T, Letzel T,
Sakuda S, Fukamizo T. Transglycosylation reaction catalyzed by a class V chitinase from cycad, Cycas revoluta: A study involving site-directed mutagenesis, HPLC, and real-time ESI-MS.Biochim Biophys Acta. 2010 Apr;1804(4):668-75.
Thammasirirak S, Pukcothanung Y, Preecharram S, Daduang S, Patramanon R,
Fukamizo T, Araki T. Antimicrobial peptides derived from goose egg white lysozyme. Comp Biochem Physiol C Toxicol Pharmacol. 2010 Jan;151(1):84-91.
Taira T, Hayashi H, Tajiri Y, Onaga S, Uechi G, Iwasaki H, Ohnuma T, Fukamizo T. A plant class V chitinase from a cycad (Cycas revoluta): biochemical characterization, cDNA isolation, and posttranslational
modification. Glycobiology. 2009 Dec;19(12):1452-61.
Dennhart N, Weigang LM, Fujiwara M, Fukamizo T, Skriver K, Letzel T. 26kDa endochitinase from barley seeds: real-time monitoring of the enzymatic reaction and substrate binding experiments using electrospray ionization mass spectrometry. J Biotechnol. 2009 Sep 25;143(4):274-83.
Takenaka Y, Nakano S, Tamoi M, Sakuda S, Fukamizo T. Chitinase gene expression in response to environmental stresses in Arabidopsis
thaliana: chitinase inhibitor allosamidin enhances stress tolerance. Biosci Biotechnol Biochem. 2009 May;73(5):1066-71.
Fukamizo T, Miyake R, Tamura A, Ohnuma T, Skriver K, Pursiainen NV, Juffer AH. A flexible loop controlling the enzymatic activity and specificity in a glycosyl hydrolase family 19 endochitinase from barley seeds (Hordeum vulgare L.). Biochim Biophys Acta. 2009 Aug;1794(8):1159-67.
Lacombe-Harvey ME, Fukamizo T, Gagnon J, Ghinet MG, Dennhart N, Letzel T, Brzezinski R. Accessory active
site residues of Streptomyces sp. N174 chitosanase: variations on a common theme in the lysozyme superfamily. FEBS J. 2009 Feb;276(3):857-69.
Fukamizo T, Hayashi K, Tamoi M, Fujimura Y, Kurotaki H, Kulminskaya A, Kitaoka M. Enzymatic
hydrolysis of 1,3-1,4-beta-glucosyl oligosaccharides by 1,3-1,4-beta-glucanase
from Synechocystis PCC6803: a comparison with assays using polymer and chromophoric oligosaccharide
substrates. Arch Biochem Biophys. 2008 Oct 15;478(2):187-94.
Dennhart N, Fukamizo T, Brzezinski R, Lacombe-Harvey ME, Letzel T. Oligosaccharide hydrolysis
by chitosanase enzymes monitored by real-time electrospray ionization-mass
spectrometry. J Biotechnol. 2008 Apr 30;134(3-4):253-60.
Kawamura S, Chijiiwa Y, Minematsu T, Fukamizo T, Vårum KM, Torikata T. The role of Arg114 at subsites E and F in reactions catalyzed by hen egg-white lysozyme. Biosci Biotechnol Biochem. 2008 Mar;72(3):823-32.
Mizuno R, Fukamizo T, Sugiyama S, Nishizawa Y, Kezuka Y,
Nonaka T, Suzuki K, Watanabe T.
Role of the Loop Structure of the Catalytic Domain in Rice Class I Chitinase.
J
Biochem. in press (2008)
Tamoi M,
Kurotaki H, Fukamizo T.
Beta-1,4-glucanase-like protein from the cyanobacterium Synechocystis PCC6803 is a beta-1,3-1,4-glucanase and functions in salt stress tolerance.
Biochem J., 405,
139-146. (2007)
Sasaki C,
Varum KM, Itoh Y, Tamoi M, Fukamizo T.
Rice chitinases: sugar recognition specificities of the individual subsites.
Glycobiology. 16,
1242-1250. (2006)
Fukamizo
T, Fleury A, Cote N, Mitsutomi M, Brzezinski R.
Exo-beta-D-glucosaminidase from Amycolatopsis orientalis: catalytic residues,
sugar recognition specificity, kinetics, and synergism.
Glycobiology. 16, 1064-1072.
(2006)
Takeuchi T, Morita K, Saito T, Kugimiya W, Fukamizo T.
Chitosan-soyprotein interaction as determined by thermal unfolding experiments.
Biosci Biotechnol Biochem. 70, 1786-1789. (2006)
Cote N, Fleury A, Dumont-Blanchette E, Fukamizo T, Mitsutomi
M, Brzezinski R.
Two exo-beta-D-glucosaminidases/exochitosanases from actinomycetes define
a new subfamily within family 2 of glycoside hydrolases.
Biochem J. 394, 675-686. (2006)
Katsumi T,
Lacombe-Harvey ME, Tremblay H, Brzezinski R, Fukamizo
T.
Role of acidic amino acid residues in chitooligosaccharide-binding to Streptomyces sp. N174 chitosanase.
Biochem
Biophys Res Commun. 338, 1839-1844.
(2005)
Fukamizo
T, Amano S,
Yamaguchi K, Yoshikawa T, Katsumi T, Saito J, Suzuki M, Miki K, Nagata Y, Ando
A. Bacillus circulans
MH-K1 chitosanase: amino acid residues responsible for substrate binding. J Biochem. 138,
563-569. (2005)
Ohnishi T,
Juffer AH, Tamoi M, Skriver K, Fukamizo T.
26 kDa endochitinase from barley seeds: an interaction of the ionizable side
chains essential for catalysis. J
Biochem. 138, 553-562. (2005)
Tamoi, M., Miyazaki, T., Fukamizo, T., and Shigeoka, S.: The Calvin cycle in cyanobacteria is regulated by
CP12 via the NAD(H)/NADP(H) ratio under light/dark conditions. Plant J. 42, 504-13 (2005)
Kojima, M., Yoshikawa, T., Ueda, M., Nonomura, T., Matsuda, Y., Toyoda,
H., Miyatake, K., Arai, M., Fukamizo, T.:
Family 19 chitinase from Aeromonas sp. No.10S-24; role of chitin-binding
domain in the enzymatic activity. J.
Biochem. 137, 235-242
(2005)
Watanabe T, Hashimoto Y, Umemoto Y, Tatebe D, Furuta E, Fukamizo T, Yamamoto K,
Utsumi R.Molecular characterization of the
essential response regulator protein YycF in Bacillus subtilis. J. Mol. Microbiol. Biotechnol. 6, 155-63 (2003)
Sasaki C, Kristiansen A, Fukamizo T, Varum KM. Biospecific fractionation of chitosan. Biomacromolecules. 4, 1686-1690. (2003)
Jaques AK, Fukamizo T, Hall D,
Barton RC, Escott GM, Parkinson T, Hitchcock CA, Adams DJ. Disruption of the
gene encoding the ChiB1 chitinase of Aspergillus fumigatus and characterization
of a recombinant gene product. Microbiology. 149, 2931-2939
(2003)
Kawamura S, Fukamizo T, Araki T, Torikata T. Expression of a synthetic
gene coding for ostrich egg-white lysozyme in Pichia pastoris and its enzymatic activity. J Biochem 133,
123-31. (2003)
Ueda, M., Kojima, M., Yoshikawa, T., Mitsuda, N., Araki, K., Kawaguchi,
T., Miyatake, K., Arai, M., Fukamizo, T.: A novel type of family 19
chitinase from Aeromonas sp. No.10S-24; cloning, sequence, expression,
and the enzymatic properties. Eur.
J. Biochem. 270, 2513-2520 (2003)
Sasaki, C., Itoh, Y., Takehara, H., Kuhara, S. and Fukamizo, T.: Family
19 chitinases from rice (Oryza sativa L.): substrate-binding subsites
demonstrated by kinetic and molecular modeling studies. Plant Mol. Biol. 52, 43-52
(2003)
Honda, Y., Kitaoka, M., Tokuyasu, K., Sasaki, C., Fukamizo, T., Hayashi,
K.: Kinetic studies on the hydrolysis of N-acetylated and N-deacetylated
derivatives of 4-methylumbelliferyl chitobiosides by the family 18 chitinases
ChiA and ChiB from Serratia marcescens.
J. Biochem. 133, 253-258 (2003)
Tanabe, T., Morinaga, K., Fukamizo, T. and Mitsutomi, M.: Novel
chitosanase from Streptomyces griseus HUT 6037 with transglycosylation
activity. Biosci. Biotech. Biochem.
67, 354-364 (2003)
Kawamura, S., Fukamizo, T., Araki, T. and Torikata, T.: Expression of a
synthetic gene coding for ostrich egg-white lysozyme in Pichia pastoris
and its enzymatic activity.: J. Biochem. 133, 123-131 (2003)
Zhang, H., Huang, X., Fukamizo, T., Muthukrishnan, S. and Kramer, K.J.:
Site-directed mutagenesis and functional analysis of an active site tryptophan
of insect chitinase. Insect Biochem.
Mol. Biol. 32, 1477-1488 (2002)
Sasaki, C., Yokoyama, A., Itoh, Y., Hashimoto, M., Watanabe, T., and Fukamizo, T. Comparative study of the
reaction mechanism of family 18 chitinases from plants and microbes. J. Biochem. 131,
557-564. (2002)
Blanchard, J., Fukamizo, T., Boucher, I., and Brzezinski, R. High level expression of the chitosanase
from Streptomyces sp. N174 and study of its intramolecular
interactions. Recent Res. Devel.
Microbiology 5, 111-121 (2001)
Tremblay, H., Yamaguchi, T., Fukamizo, T. and Brzezinski, R. Mechanism of
Chitosanase-Oligosaccharide Interaction: Subsite Structure of Streptomyces sp. N174 Chitosanase and
the role of Asp57 carboxylate. J. Biochem. 130, 679-686 (2001)
Zhu, X., Zhang, H., Fukamizo,
T., Muthukrishnan, S. and Kramer, K.J.
Properties of Manduca sexta chitinase and its C-terminal deletions. Insect Biochem. Mol. Biol., 31,
1221-1230 (2001)
Fukamizo, T., Yamaguchi, T., Araki,
T., Torikata, T., Kristiansen, A., and Varum, K.M.: Binding of a highly de-N-acetylated
chitosan to Japanese pheasant lysozyme as measured by 1H-NMR
spectroscopy. Biosci. Biotech.
Biochem. 65, 1776-1773 (2001)
Fukamizo, T.,
Sasaki, C., Schelp, E., Bortone, K., and Robertus, J.D.: Kinetic properties of
chitinase-1 from the fungal pathogen Coccidioides immitis. Biochemistry 40, 2448-2454 (2001)
Fukamizo, T.,
Juffer, A.H., Vogel, H.J., Honda, Y., Tremblay, H., Boucher, I., Neugebauer,
W.A., and Brzezinski, R.: Theoretical calculation of pKa reveals an important
role of Arg205 in the activity and stability of Streptomyces sp. N174
chitosanase. J. Biol. Chem. 275,
25633-25640 (2000)
Fukamizo, T.:
Chitinolytic enzymes: Catalysis, substrate binding, and their application. Curr. Protein & Peptide Sci. 1,
105-124 (2000)
Honda,
Y., Tanimori, S., Kirihata, M., Kaneko, S., Tokuyasu, K., Hashimoto, M., Watanabe,
T., and Fukamizo, T.: Kinetic analysis of the reaction catalyzed by
chitinase A1 from Bacillus circulans WL-12 toward the novel substrates,
partially N-deacetylated 4-methylumbelliferyl chitobiosides. FEBS Lett. 476, 194-197 (2000)
Honda, Y., Kirihata, M., Fukamizo, T.,
Kaneko, S., Tokuyasu, K., and Brzezinski, R.: Chitosanase-catalyzed hydrolysis
of 4-methylumbelliferyl b-chitotrioside.
J. Biochem. 126, 470-474 (1999)
Honda, Y., Fukamizo, T., Okajima, T.,
Goto, S., Boucher, I., and Brzezinski, R.: Thermal unfolding of chitosanase
from Streptomyces sp. N174: role of tryptophan residues in the protein
structure stabilization. Biochim.
Biophys. Acta 1429, 365-376 (1999)
Okajima, T., Fukamizo, T.,
Goto, S., Fukui, T., and Tanizawa, T. Exchange of nucleotide monophosphate
binding domain in adenylate kinase and UMP/CMP kinase. J. Biochem. 124, 359-367
(1998)
Honda, Y. and Fukamizo, T. Substrate Binding Subsites of Chitinase from
Barley Seeds and Lysozyme from Goose Egg White: Biochim. Biophys.Acta 1388,
53-65 (1998)
Fukamizo, T. and Brzezinski,
R. Chitosanase from Streptomyces sp. N174:
A Comparative review of Its Structure and Function. Biochem. Cell Biol. 75, 687-696 (1997)
Hollis, T., Honda, Y., Fukamizo, T.,
Marcotte, E.M., and Robertus, J.D. Kinetic analysis of barley chitinase. Arch. Biochem. Biophys. 344,
335-342 (1997)
Honda, Y., Fukamizo, T.,
Boucher, I. and Brzezinski, R. Substrate Binding to the Inactive Mutants of
Streptomyces sp.N174 Chitosanase: Indirect Evaluation from the Thermal
Unfolding Experiments. FEBS Lett.
411, 346-350 (1997)
Fukamizo, T., Honda, Y.,
Toyoda, H., Ouchi, S., and Goto, S.：Chitinous component of the cell wall of Fusarium
oxysporum. Its structure deduced from chitosanase digestion. Biosci.
Biotech. Biochem. 60(10), 1705-1708 (1996)
Boucher, I., Fukamizo, T.,
Honda, Y., Willick, Neugebauer, W. A. and Brzezinski, R.：
Site-directed mutagenesis of evolutionary conserved carboxylic amino acids in
the chitosanase from Streptomyces sp. N174 reveals two residues
essential for catalysis. J. Biol. Chem. 270, 31077-31082 (1995)
Fukamizo, T., Honda, Y., Goto,
S., Boucher, I., and Brzezinski, R.：Reaction mechanism of chitosanase from Streptomyces
sp. N174. Biochem. J. 311, 377-383 (1995)
Fukamizo, T., Hatta, K., and
Goto, S.：Hen egg white lysozyme
modified with histamine. State of the imidazolylethyl group covalently attached
to the binding site and its effect on the sugar binding ability. Eur. J.
Biochem. 231, 56-64 (1995)
Fukamizo, T., Koga, D., and
Goto, S.：Comparative
biochemistry of chitinases-Anomeric form of the reaction products. Biosci.
Biotech. Biochem. 59(2), 311-313 (1995)
Fukamizo, T., Ohkawa, T.,
Ikeda, Y., Torikata, T., and Goto, S.：Binding
mode of N, N, N, N-Tetraacetylchitotetraitol to hen egg white lysozyme. Carbohydr.
Res. 267, 135-142 (1995)
Fukamizo,T., Ohkawa, T.,
Ikeda, Y., and Goto, S.：Specificity
of chitosanase from Bacillus pumilus. Biochim. Biocim. Boiphys. Acta
1205/ 183-188 (1994)
Toyoda, H., Morimoto, Kakutani, Morikawa, Fukamizo, T., Goto, S., Terada, Ouchi, S.：Binary
Microbe System for Biological Control of Fusarium Wilt of Tomato;
Enhanced Root Colonization of an Antifungal Rhizoplane acteriumbSupported by a
Chitin-degrading Bacterium. Ann. Phytopathol. Soc. Japan 59(4),
375-386 (1993)
Fukamizo, T., Ohkawa, T.,
Ikeda, Y., Goto, S.：1H-NMR
Study on the Chitotrisaccharide Binding to en egg While Lysozyme.：Eur.
J. Biochem. 210, 351-357 (1992)
Fukamizo,
T., Ohkawa, T., Sonoda, K., Toyoda, H., Nishiguchi, Ouchi, S., Goto, S.：hitinous Components of the cell wall of Fusarium
oxysporum：Biosci.
Biotech. Biochem. 56(10),
1632-1636 (!992)
To-Anun, Toyoda, H., Nishiguchi, Chang, Fukamizo, T., Ouchi, S.：Isolatiom
and regeneration of Fusarium oxysporum f. sp. lycopersici
Protoplasts：Ann.
Phytopathol. Soc. Japan
57(4), 609-612 (1991)
Fukamizo,
T., Ikeda, Y., Torikata, T., Araki, T., Kuramoto, M., Goto, S.：1H-NMR
study on the structure of lysozyme from guinea hen egg white：J. Biochem. 110, 997-1003 (1991)
Fukamizo,
T., Ohtakara, A., Mitsutomi, M., Goto, S.：NMR spectra of partially deacetylated
chitotrisaccarides：Agric.
Biol. Chem.. 55(10),
2653-2655 (!991)
Fukamizo,
T., Goto, S.：Lysozyme-catalyzed
reaction in continuous flow system：J. Biochem. 109, 416-420 (1991)
Fukamizo,
T., Sonoda, Goto, S., Toyoda, H., Ouchi, S.：Solid State 13C-NMR analysis of cell wall
from Fusarium oxysporum：Agric.
Biol. Chem. 54(10),
2761-2762 (1990)
Mitsutomi, M., Ohtakara, A., Fukamizo, T., Goto, S.：Action pattern of Aeromonas hydrophila
chitinase on partially N-acetylated chitosan：Agric, Biol. Chem. 54(4), 871-877 (1990)
Fukamizo,
T., Goto, S., Torikata, T., Araki, T.：State of binding subsites in Asp 101-modified lysozyme：J. Biochem. 107,
445-451 (1990)
Fukamizo,
T., Goto, S., Torikata, T., Araki, T.：Enhancement of transglycosylation activity of lysozyme
by chemical modification：Agric.
Biol. Chem. 53(10),
2641-2651 (1989)
Yanase, Y., Fukamizo,
T., Hayashi, K., Goto, S.：Retention
of anomeric form in lysozyme-catalyzed reaction：Arch. Biochem. Biophy. 253(1), 168-175 (1987)
Fukamizo,
T., Kramer, K. J.：Effect
of 20-hydroxyedysone on chitinase and b-N-acetylglucosaminidase during
thr larval-pupal transformation of Manduca sexta：Insect Biochem. 17(4), 547-550 (1978)
Fukamizo,
T., Minematsu, T., Yanase, Y., Hayashi, K., Goto, S.：Substrate size dependence of lysozyme-catalyzed
reaction：Arch.
Biochem. Biophys. 250(2),
312-321 (1986)
Fukamizo,
T., Kramer, K. J., Mueller, D. D., Schaefer, J., Garbow, Jacob,：Analysis of chitin structure by nuclear magnetic
resonance spectroscopy and chitinolytic enzyme digestion：Arch. Biochem. Biophys. 249(1), 15-26 (1986)
Fukamizo,
T., Hayashi, K., Goto, S.：The
role of binding subsite A in reactions catalyzed by hen egg white lysozyme：Eur. J. Biochem. 158, 463-467 (1986)
Fukamizo,
T., Speirs, R. D., Kramer, K. J.：Conparative biochemistry of mycophagous and
nonmycophagous grain beetles. Chitinolytic activities of foreign and sawtoothed
grain beetles：Comp.
Biochem. Physiol. 81B(1), 207-209(1985)
Fukamizo,
T., Kramer, K. J.：Mechanism
of chitin hydrolysis by the binary chitinase system in insect molting fluid：Insect Biochem. 15(2), 141-145 (1985)
Fukamizo,
T., Kramer, K.J.：Mechanism
of chitin oligosaccharide hydrolysis by the binary enzyme chitinase system in
insect molting fluid：Insect
Biochem. 15(1),
1-7 (1985)
Fukamizo,
T., Torikata, T., Nagayama, T., Minematsu, T., Hayashi, K.：Enzymatic activity of avian egg-white lysozymes：J. Biochem. 94, 115-122 (1983)
Fukamizo,
T., Kuhara, S., Hayashi, K.：Enzymatic
activity of Trp62-modified lysozyme：J. Biochem. 92, 717-724 (1982)
Fukamizo,
T., Kuhara, S., Hayashi, K.：Human
lysozymr-catalyzed reactiom of chitooligosaccarides：J. Biochem. 92, 709-716 (1982)
Kuhara, S., Ezaki, Fukamizo. T., Hayashi, K：Estimation of the binding free energy changes in
lysozyme-catalyzed reaction：J.
Biochem. 91,
121-127 (1982)
Fukamizo,
T., Hayashi, K.：Separation
and mutarotation of anomers of chitooligosaccarides：J. Biochem. 91, 619-626 (1982)
Masaki, A., Fukamizo,
T., Otakara, A., Torikata, T., Hayashi, K., Imoto, T.：Estimation of rate constants in lysozyme-catalyzed
reaction of chitooligosaccarides：J. Biochem. 90, 1167-1175 (1981)
Masaki, A., Fukamizo,
T., Otakara, A., Torikata, T., Hayashi, K., Imoto, T.：Lysozyme-catalyzed
reaction of chitooligosaccarides：J. Biochem. 90, 527-533 (1981)